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resource source identifier sheep anti-mouse reg3β  (R&D Systems)


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    R&D Systems resource source identifier sheep anti-mouse reg3β
    Resource Source Identifier Sheep Anti Mouse Reg3β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+reg3%CE%B2/Mouse+Reg3B+Antibody/10__2139_slash_ssrn__3895626-621-5-13
    Average 91 stars, based on 20 article reviews
    resource source identifier sheep anti-mouse reg3β - by Bioz Stars, 2026-09
    91/100 stars

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    Article Title: Lipid malabsorption from altered hormonal signaling changes early gut microbial responses
    Article Snippet: Primary antibodies were as follows: goat anti-Sst (Santa Cruz Biotechnology, sc7819), rabbit anti-chromogranin A (CgA) (Abcam, ab15160), mouse anti-Glp1 (Abcam, ab26278), goat anti-Cck (Santa Cruz, sc21617), goat anti-Gip (Santa Cruz, sc23554), rabbit anti-lysozyme (Abcam ab108508), goat anti-Sct (Santa Cruz, sc21023), mouse anti-Reg3β (R&D Systems, AF5110), mouse anti-E-cadherin (BD Transduction, 610181), goat anti-peptide tyrosine tyrosine (Pyy) (Santa Cruz, sc47318), and rabbit anti-myeloperoxidase (Mpo) (Agilent Technologies, A039829-2).



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    Representative images of murine SI (a), murine colon (b) or human colon (c) organoids either unstimulated or stimulated with 500U/ml of IFNβ for 48 h and expression of Mx2 mRNA relative to unstimulated organoids. Scale bars represent 50μM. The following number of independent experiments were performed: (a), n=7; (b), n=3 and (c), n=3. Log 10 transformed Mx2 expression was analyzed by two-tailed paired t-test. (d) Phosphorylation of STAT3 at 2 h in SI organoids. Representative of 4 independent experiments. (e) Representative images of <t>Reg3β</t> expression and representative mean fluorescence intensity (MFI) of individual organoids of 2 independent experiments at 24 h. Scale bars represent 50μM. The following number of organoids were analyzed: unstimulated, n=34; IFNβ, n=32 and IL-22, n=40. ANOVA with Dunnett’s multiple comparisons test was used to analyze Reg3β expression. Proportion of IL-22 expressing ILC (f) and CD11b low CD11c + CD103 − cells (g) in ABX-treated Ifnar1 f/f and Ifnar1 ΔIEC mice at day 6 post DSS. The following number of mice were analyzed for IL-22: Ifnar1 f/f ABX, n=16; Ifnar1 f/f ABX+CR6, n=19; Ifnar1 ΔIEC ABX, n=15 and Ifnar1 ΔIEC ABX+CR6, n=15. The following number of mice were analyzed for CD11b low CD11c + CD103 − cells: Ifnar1 f/f ABX, n=18; Ifnar1 f/f ABX+CR6, n=20; Ifnar1 ΔIEC ABX, n=14 and Ifnar1 ΔIEC ABX+CR6, n=14. ANOVA with Tukey’s multiple comparisons test was used to analyze cell populations. (h) Gene expression analysis of IECs from MNV CR6-infected Ifnar1 f/f (n=3) and Ifnar1 ΔIEC (n=3) mice compared to uninfected mice ( Ifnar1 f/f , n=3 and Ifnar1 ΔIEC , n=3) at day 6 post DSS. Circles in Venn diagram represent number of transcripts that are enriched following MNV-infection in the mice with the indicated genotypes. Pathway analysis was performed on the non-overlapping gene set and p-values determined by Ingenuity Pathway Analysis. All bars represent mean and error bars represent standard error of the mean. All p-values are shown in the figure.
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    Representative images of murine SI (a), murine colon (b) or human colon (c) organoids either unstimulated or stimulated with 500U/ml of IFNβ for 48 h and expression of Mx2 mRNA relative to unstimulated organoids. Scale bars represent 50μM. The following number of independent experiments were performed: (a), n=7; (b), n=3 and (c), n=3. Log 10 transformed Mx2 expression was analyzed by two-tailed paired t-test. (d) Phosphorylation of STAT3 at 2 h in SI organoids. Representative of 4 independent experiments. (e) Representative images of <t>Reg3β</t> expression and representative mean fluorescence intensity (MFI) of individual organoids of 2 independent experiments at 24 h. Scale bars represent 50μM. The following number of organoids were analyzed: unstimulated, n=34; IFNβ, n=32 and IL-22, n=40. ANOVA with Dunnett’s multiple comparisons test was used to analyze Reg3β expression. Proportion of IL-22 expressing ILC (f) and CD11b low CD11c + CD103 − cells (g) in ABX-treated Ifnar1 f/f and Ifnar1 ΔIEC mice at day 6 post DSS. The following number of mice were analyzed for IL-22: Ifnar1 f/f ABX, n=16; Ifnar1 f/f ABX+CR6, n=19; Ifnar1 ΔIEC ABX, n=15 and Ifnar1 ΔIEC ABX+CR6, n=15. The following number of mice were analyzed for CD11b low CD11c + CD103 − cells: Ifnar1 f/f ABX, n=18; Ifnar1 f/f ABX+CR6, n=20; Ifnar1 ΔIEC ABX, n=14 and Ifnar1 ΔIEC ABX+CR6, n=14. ANOVA with Tukey’s multiple comparisons test was used to analyze cell populations. (h) Gene expression analysis of IECs from MNV CR6-infected Ifnar1 f/f (n=3) and Ifnar1 ΔIEC (n=3) mice compared to uninfected mice ( Ifnar1 f/f , n=3 and Ifnar1 ΔIEC , n=3) at day 6 post DSS. Circles in Venn diagram represent number of transcripts that are enriched following MNV-infection in the mice with the indicated genotypes. Pathway analysis was performed on the non-overlapping gene set and p-values determined by Ingenuity Pathway Analysis. All bars represent mean and error bars represent standard error of the mean. All p-values are shown in the figure.
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    Image Search Results


    Representative images of murine SI (a), murine colon (b) or human colon (c) organoids either unstimulated or stimulated with 500U/ml of IFNβ for 48 h and expression of Mx2 mRNA relative to unstimulated organoids. Scale bars represent 50μM. The following number of independent experiments were performed: (a), n=7; (b), n=3 and (c), n=3. Log 10 transformed Mx2 expression was analyzed by two-tailed paired t-test. (d) Phosphorylation of STAT3 at 2 h in SI organoids. Representative of 4 independent experiments. (e) Representative images of Reg3β expression and representative mean fluorescence intensity (MFI) of individual organoids of 2 independent experiments at 24 h. Scale bars represent 50μM. The following number of organoids were analyzed: unstimulated, n=34; IFNβ, n=32 and IL-22, n=40. ANOVA with Dunnett’s multiple comparisons test was used to analyze Reg3β expression. Proportion of IL-22 expressing ILC (f) and CD11b low CD11c + CD103 − cells (g) in ABX-treated Ifnar1 f/f and Ifnar1 ΔIEC mice at day 6 post DSS. The following number of mice were analyzed for IL-22: Ifnar1 f/f ABX, n=16; Ifnar1 f/f ABX+CR6, n=19; Ifnar1 ΔIEC ABX, n=15 and Ifnar1 ΔIEC ABX+CR6, n=15. The following number of mice were analyzed for CD11b low CD11c + CD103 − cells: Ifnar1 f/f ABX, n=18; Ifnar1 f/f ABX+CR6, n=20; Ifnar1 ΔIEC ABX, n=14 and Ifnar1 ΔIEC ABX+CR6, n=14. ANOVA with Tukey’s multiple comparisons test was used to analyze cell populations. (h) Gene expression analysis of IECs from MNV CR6-infected Ifnar1 f/f (n=3) and Ifnar1 ΔIEC (n=3) mice compared to uninfected mice ( Ifnar1 f/f , n=3 and Ifnar1 ΔIEC , n=3) at day 6 post DSS. Circles in Venn diagram represent number of transcripts that are enriched following MNV-infection in the mice with the indicated genotypes. Pathway analysis was performed on the non-overlapping gene set and p-values determined by Ingenuity Pathway Analysis. All bars represent mean and error bars represent standard error of the mean. All p-values are shown in the figure.

    Journal: Nature microbiology

    Article Title: IFN-I and IL-22 mediate protective effects of intestinal viral infection

    doi: 10.1038/s41564-019-0470-1

    Figure Lengend Snippet: Representative images of murine SI (a), murine colon (b) or human colon (c) organoids either unstimulated or stimulated with 500U/ml of IFNβ for 48 h and expression of Mx2 mRNA relative to unstimulated organoids. Scale bars represent 50μM. The following number of independent experiments were performed: (a), n=7; (b), n=3 and (c), n=3. Log 10 transformed Mx2 expression was analyzed by two-tailed paired t-test. (d) Phosphorylation of STAT3 at 2 h in SI organoids. Representative of 4 independent experiments. (e) Representative images of Reg3β expression and representative mean fluorescence intensity (MFI) of individual organoids of 2 independent experiments at 24 h. Scale bars represent 50μM. The following number of organoids were analyzed: unstimulated, n=34; IFNβ, n=32 and IL-22, n=40. ANOVA with Dunnett’s multiple comparisons test was used to analyze Reg3β expression. Proportion of IL-22 expressing ILC (f) and CD11b low CD11c + CD103 − cells (g) in ABX-treated Ifnar1 f/f and Ifnar1 ΔIEC mice at day 6 post DSS. The following number of mice were analyzed for IL-22: Ifnar1 f/f ABX, n=16; Ifnar1 f/f ABX+CR6, n=19; Ifnar1 ΔIEC ABX, n=15 and Ifnar1 ΔIEC ABX+CR6, n=15. The following number of mice were analyzed for CD11b low CD11c + CD103 − cells: Ifnar1 f/f ABX, n=18; Ifnar1 f/f ABX+CR6, n=20; Ifnar1 ΔIEC ABX, n=14 and Ifnar1 ΔIEC ABX+CR6, n=14. ANOVA with Tukey’s multiple comparisons test was used to analyze cell populations. (h) Gene expression analysis of IECs from MNV CR6-infected Ifnar1 f/f (n=3) and Ifnar1 ΔIEC (n=3) mice compared to uninfected mice ( Ifnar1 f/f , n=3 and Ifnar1 ΔIEC , n=3) at day 6 post DSS. Circles in Venn diagram represent number of transcripts that are enriched following MNV-infection in the mice with the indicated genotypes. Pathway analysis was performed on the non-overlapping gene set and p-values determined by Ingenuity Pathway Analysis. All bars represent mean and error bars represent standard error of the mean. All p-values are shown in the figure.

    Article Snippet: Staining was performed using sheep anti-mouse Reg3β antibody (R&D systems) followed by detection with anti-sheep Ig NL557 (R&D systems) and mounting with Vectashield containing Dapi (Vector Laboratories).

    Techniques: Expressing, Transformation Assay, Two Tailed Test, Phospho-proteomics, Fluorescence, Gene Expression, Infection